Coding

Part:BBa_K5102066:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-09-30)


Tetracycline-controlled transactivator (tTA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part was obtained from the lab, the design considerations focused on ensuring proper incorporation into plasmids for expression and verifying compatibility with BioBrick requirements.


Source

The part has been amplified from an already existing plasmid in the lab.

References